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    2020-08-28


    Contents lists available at ScienceDirect
    European Journal of Integrative Medicine
    journal homepage: www.elsevier.com/locate/eujim
    Research paper
    Carlina curetum plant phytoconstituents, enzymes inhibitory and cytotoxic T activity on cervical epithelial carcinoma and colon cancer cell lines
    Nidal Amin Jaradata, , Saad Al-lahhamb, Abdel Naser Zaida, Fatima Husseina, Linda Issaa, Murad N Abualhasana, Mohammed Hawasha, Amjad Yahyaa, Omar Shehadia, Rashed Omaira, Ahmad Mousab
    a Department of Pharmacy, Faculty of Medicine and Health Sciences, An-Najah National University, 00970, Nablus, Palestine
    b Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, An-Najah National University, 00970, Nablus, Palestine
    Keywords:
    Carlina curetum α-amylase α-glucosidase Anti-Lipase
    Cytotoxicity
    HeLa
    Introduction: Plant products are a rich source of pharmacologically active molecules and are considered an important and attractive field of scientific investigation for the development of new drugs. The present study aimed to assess the α-amylase, α-glucosidase and AB7FUBAICA lipase enzymes inhibitory activities and the cytotoxic effects of Carlina curetum four solvents fractions in order to better understand the anti-obesity, hypoglycemic and antic-ancer effects of this plant.
    Methods: Several qualitative and quantitative phytochemical tests were performed on Carlina curetum solvent fractions utilizing standard phytochemical procedures, followed by an investigation into their ability to inhibit the enzymes α-amylase, α-glucosidase, and lipase and an assessment of cytotoxic activity against HeLa and Colo-205 AB7FUBAICA using standard biochemical and biotechnological methods.
    Results: The results revealed that the aqueous and methanol fractions had the highest α-amylase enzyme in-hibitory activity with IC50 values of 21.37 ± 0.31 and 30.2 ± 0.42 μg/mL, respectively, in comparison with Acarbose, which had an IC50 value of 28.18 ± 0.42 μg/mL. The methanol fraction showed potent α-glucosidase inhibitory activity with an IC50 value of 27.54 ± 4.28 μg/mL; the α-glucosidase inhibitory activity Acarbose was 37.15 ± 0.33 μg/mL. The hexane fraction had greater anti-lipase activity than Orlistat. In addition, 0.5 mg/ mL of the C. curetum acetone and hexane fractions had pronounced cytotoxic effects on the Colo-205 cancer cell line, and 0.625 mg/ml of the C. curetum hexane fraction had potential cytotoxic effects against the cervical epithelial carcinoma (HeLa) cell line.
    Conclusion: This study revealed that C. curetum has potential α-amylase, α-glucosidase, porcine pancreatic lipase enzyme inhibitory activity and cytotoxic activity against the HeLa and Colo-205 cancer cell lines, which in-dicates the presence of biologically active and cytotoxic compounds in this plant species. This may be considered a challenge for developing bioactive compounds in diabetes, obesity and cancer management.
    1. Introduction
    For centuries, plants, animals and other natural products have been considered an endless and valuable source of therapeutic activities. Certainly, a vast number of drugs have been isolated from plants or their semisynthetic molecules [1,2]. However, investigations into nat-ural products and their isolated derivatives are associated with some basic difficulties, such that most pharmaceutical companies shifted their fundamental focus toward purified synthetic or semisynthetic chemical compounds [3]. Unfortunately, chemical medications do not meet the expectations of patients, pharmaceutical companies or global
    Corresponding author.
    E-mail address: [email protected] (N.A. Jaradat). 
    health organizations, as evidenced by decreasing numbers of novel medications reaching the pharmaceutical market and unexpected harmful side effects, contraindications and drug-drug interactions [4–6]. For these reasons, pharmaceutical company interests have moved again toward natural product-based drugs, and many broad interdisciplinary and multidisciplinary investigations into natural pro-ducts have been carried out [7]. A commonly used approach is to start pharmacological investigations with crude plant extracts and thereafter isolate and characterize the phytoconstituents responsible for the pharmacological effect [8–11].
    Recently, it has been clearly shown that cancer, diabetes, and
    obesity are major crises and challenges to global health care organi-zations and pharmaceutical research centers in the 21st century [12]. There is a scientifically supported relationship between cancer, obesity and diabetes mellitus, such that many obese patients may also have diabetes cancer [13].